The aim of the present research work was to investigate the wound healing effect of Psidium guajava leaves extract. Psidium guajava Linn (Myrtaceae) has been used as traditional medicine to treat wound. We investigated the wound healing mechanisms of Psidium guajava Leaves Extract. Hydroalcoholic and methanolic extract of Psidium guajava leaves was used. The comparative study of hydroalcoholic and methanolic extract of Psidium guajava leaves was carried out to evaluate wound healing activity. The major findings of the study demonstrated the significant wound healing potential of Psidium guajava leaves extract gel formulation. Clinical healing was established by measuring the area of the wound in each test group on days 3, 6, 9 and 12. Hydroalcoholic extract and methanolic extract containing gel applications result decrease in the wound area in 12 days. Comparative study was done in hydroalcoholic extract and methanolic extract. Hydroalcoholic extract is more effective in comparision with methanolic extract. Histological evaluation showed that the Psidium guajava leaves extract group had a high histological score than the control groups during the whole test period and the difference between the Psidium guajava leaves extract group and the control group was significant. Statistical differences between means were determined by oneway ANOVA followed by Turkey’s test. Values of p<0.040, were considered as significant in this experimental animal study. Formulated herbal gel containing methanolic and hydroalcoholic extract test groups and standerd which indicates that formulated herbal gel was as effective as standard gel for wound healing activity. From the results it was concluded that Psidium guajava leaves extract when formulated in gel show significant improvement in wound contraction and hence this is a promising candidate for wound healing activity. Formulated herbal gel containing methanolic and hydroalcoholic extract test groups and standard which indicates that formulated herbal gel was as effective as standard gel for wound healing activity.
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